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เพาะเลี้ยงเนื้อเยื่อไม่ยาก ค่าอุปกรณ์ราคาประหยัด!!!

อุปกรณ์การเกษตรเพาะเลี้ยงเนื้อเยื่อ

สืบเนื่องมาจากกอกล้วยหน้าบ้านตกเครือเป็นครั้งแรก สร้างความตื่นเต้นให้กับ admin มาก และก็ไม่ผิดหวังเลยหลังจากที่ได้ลองชิม (กันเป็นหวีๆ) แล้ว เพราะเนื้อที่แน่น หวาน ไส้เหลืองอ่อนๆ อร่อยมากๆ อยากจะเพาะพันธ์แจกกันเลย แต่มองไปที่หน่อแล้วคงแจกได้เต็มที่ก็พี่ รปภ. ของหมู่บ้าน ( มีคนเดียว)

แว่บนั้น…พลันก็นึกขึ้นได้ถึงวิธีการขยายพันธุ์ที่เคยเห็นตอนอยู่ ม. ต้น ( ก็แค่กว่า 20 ปีก่อนเอง..อิอิ) อืม…วิธีก็ไม่ใหม่แล้ว ไหงราคาอุปกรณ์ยังเป็นหมื่นแก่ๆถึงแสนอ่อนๆอยู่เลย ทันใดนั้นก็เห็นข่าวไทยรัฐที่ลงไว้เมื่อปี 2557 ว่าเราสามารถเพาะเนื้อเยื่อพืชได้โดยลงทุนค่าอุปกรณ์แค่หลักร้อย.. น่าสนใจมาก..ไปดูกันว่าเค้าทำยังไง

คิดจะเพาะเลี้ยงเนื้อเยื่อบ้าง ไม่ใช่เรื่องยากเกินเอื้อม…น.ส.มนฑิณี กมลธรรม นักวิชาการ ฝ่ายเทคโนโลยีการเกษตร สถาบันวิจัยวิทยาศาสตร์และเทคโนโลยีแห่งประเทศไทย (วว.) ได้คิดวิธีการแบบใหม่ ลงทุนแค่ไม่กี่ร้อยบาท เราสามารถเนรมิตห้องเพาะเนื้อเยื่อทำเล่นสนุกๆภายในบ้านได้แล้ว

“การเพาะเลี้ยงเนื้อเยื่อ สิ่งสำคัญที่สุดคือความสะอาด กับใจเย็น ไม่อย่างนั้นเลี้ยงไม่รอด ไม่กี่วันอาหารเป็นเชื้อรา เนื้อเยื่อตายหมด ถ้าใครยังไม่เคยทำ ให้ลองเอากล้วยไม้มาทำก่อน เพราะง่ายที่สุด เขี่ยเนื้อเยื่อเสร็จเลี้ยงอีกแค่ 5-6 เดือน ต้นเริ่มโตก็ขายได้ หรือเอาไปเป็นของขวัญของฝากในเทศกาลต่างๆก็ไม่เลว”

วิธีการไม่ยาก อุปกรณ์ก็หาง่าย เริ่มด้วยการทำอาหารเนื้อเยื่อ

ใช้น้ำสะอาด 1 ลิตร

วุ้นทำอาหาร 8 กรัม

ถ่านไม้บด 0.2 กรัม

มันฝรั่งบด 50 กรัม

กล้วยหอมบด 50 กรัม

ปุ๋ยสูตรที่มีไนโตรเจนสูง (N) 2 กรัม

ใส่รวมในหม้อต้ม คนให้เข้ากันกระทั่งน้ำเดือด เทอาหารเนื้อเยื่อใส่ขวด สูงประมาณ 1 ซม. หยดน้ำยาซักผ้าขาวที่มีสารโซเดียมไฮโปคลอไรด์ตามทันทีประมาณ 5 หยด/ขวด…ปิดฝาอย่างรวดเร็ว ความร้อนกับน้ำยาซักผ้าขาวจะฆ่าเชื้อได้ดี

วิธีนี้เลยไม่ต้องใช้หม้อนึ่งความดันให้สิ้นเปลือง

สูตรนี้ได้อาหารเลี้ยงเนื้อเยื่อ 40-50 ขวด เมื่ออาหารเย็นจับตัวเป็นก้อน เอาไปเลี้ยงเนื้อเยื่อได้เลย ขั้นตอนนี้โดยทั่วไปจะทำกันใน “ตู้ปลอดเชื้อ” ราคาแพงๆ แต่ มนฑิณี ใช้ “ตู้เลี้ยงปลา” ล้างให้สะอาด ภายในมีตะเกียงแอลกอฮอล์…ก่อนลงมือเอาเนื้อเยื่อมาเพาะเลี้ยง ต้องใช้เอทิลแอลกอฮอล์ 70% เช็ดฆ่าเชื้อตามฝ่ามือ ขวดอาหารเนื้อเยื่อ รวมทั้งปากคีบต้องเผาไฟฆ่าเชื้อ 2-3 ครั้ง

 

เนื้อเยื่อที่ต้องการนำเพาะขยาย ทำได้ทั้งพืชล้มลุก ไม้ดอกไม้ประดับ แล้วแต่ใครจะชอบ ตัดเอาแค่ส่วนยอดอ่อนด้วยใบมีดที่คม แผลจะได้ไม่ช้ำ นำไปจุ่มในน้ำยาซักผ้าขาว 15% ฆ่าเชื้อก่อน จากนั้นนำไปใส่ในขวดอาหารเพาะเลี้ยงเนื้อเยื่อ…ฝา ปากขวดต้องอังกับตะเกียงไฟ 3-5 วินาทีก่อนปิดให้สนิท

นำขวดที่มีเนื้อเยื่อไปวางไว้ที่อุณหภูมิ 25–28 องศา เปิดไฟให้แสงสว่างวันละ 8 ชม. สังเกตอาหารเพาะเนื้อเยื่อหมด ย้ายต้นกล้าลงปลูกได้เลย

แต่ถ้าเป็นกล้วยไม้ ให้ย้ายต้นกล้ามาใส่กาบมะพร้าวสับ เอาไปแขวนไว้ในโรงเรือนเพาะเลี้ยง ที่แสงแดดไม่แรง 5-6 เดือน ก็สามารถเปลี่ยนเป็นเงินเข้ากระเป๋าได้สบายๆ

 

รู้อย่างนี้แล้ว ต้องลองทำกันดูนะคะ  admin ไม่พลาด (ให้สามีทำ) แน่ค่ะ อิอิ

ขอขอบคุณ http://www.thairath.co.th/content/456692

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